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Tripartite motif-containing protein 28 is a kind of macromolecular protein with E3 ubiquitin ligase, which belongs to an important member of the TRIM protein family. As a new molecular biomarker, it has attracted wide attention. TRIM28 is highly expressed in many kinds of malignant tumors, which is closely related to clinicopathological features, and is also involved in biological behaviors such as proliferation, apoptosis, migration and invasion of tumor cells. TRIM28 may be a potential marker and therapeutic target for clinical diagnosis and prognosis of tumors. This study reviews the structure and biological function of TRIM28, its relationship with malignant tumors and the molecular mechanism of signal transduction pathway.
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Objective To investigate the application value of detection of serum miRNA-210,miRNA-23b in the patients with the femoral head necrosis.Methods The serum and clinical data of 135 patients with the internal fixation operation for the femoral neck fracture were collected.The patients were divided into the necrosis group (27 cases) and non-necrosis group (108 cases),used the real-time fluorescent quantitative PCR (Real time-PCR,RT-PCR) technology to detect serum miRNA-210,miRNA-23b expression in comparatively analysizing the relationship between the changes of serum miRNA 210,23b and occurrence of the femoral head necrosis.Results The serum microRNA-210,-23b expression in the necrosis group were 0.19±0.03,0.21±0.05,which in the non-necrosis group were 0.92±0.18 and 0.87±0.17.The serum microRNA-210,-23b expression in the necrosis group were significantly lower than those in the non-necrosis group,the difference had statistical significance (P＜0.01).The analysis results from the receiver-operating characteristic curve (ROC curve) showed that AUC of serum miRNA-210,miRNA-23b expression were 0.790 (95％CI:0.687～0.892,P=0.000) and 0.743 (95％CI:0.633～0.854,P=0.000),respectively.The sensitivity and specificity of miRNA-210 and miRNA-23b at the best threshold in diagnosis of the femoral head necrosis were 95.7％ and 93.9％,91.5％ and 92.3％,respectively.The analysis results showed that the serum miRNA-210,-23b expression had clinical significance in diagnosis of the femoral head necrosis.Conclusion Detection of serum miRNA-210,-23b expression can predict occurrence of the femoral head necrosis,estimate development condition of the desease.
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Objective To comprehensively grasp the grassroots medical institutions blood transfusion(blood) management present situation,put forward improvement strategy for the universality of existence weak link,the stand-ardized management of clinical blood transfusion to strengthen grass -roots medical institutions to provide necessary theoretical support.Methods 62 grassroots medical institutions blood transfusion(blood)in Yantai district were selected as the research subjects,to set the planning,layout,basic construction standards,related technology and equipment application aspects of quantitative evaluation,preliminary conclusions.Results By examination of 62 grassroots medical institutions transfusion quality management level both in terms of hardware and software facilities, emergency plan formulation,system file support informatization construction,staff education training,etc,found many problems,there was a certain gap between the existing department of blood transfusion standards.Conclusion The current grassroots medical institutions transfusion management is conditioned by the objective conditions,there are many problems,such as not improved,will cause a certain hidden trouble on the safety of clinical blood transfusion. Therefore,should combine their own reality,strengthen the quality of blood transfusion management system construc-tion,and give full play to the supervision and management of the hospital transfusion quality management committee. At the same time,the local administrative department of health also should formulate corresponding policies,ensure transfusion management level significantly increased.
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<p><b>OBJECTIVE</b>Species containing extremely aromatic compounds in leaves of Chimonanthus was analyzed to evaluate its genetic diversity and genetic relationships.</p><p><b>METHOD</b>AFLP molecular marker technique was used in the study, UPGMA cluster analysis was conducted with the software of POPGENE32.</p><p><b>RESULTS</b>Five hundred and fifty-nine bands were amplified by 10 pairs of primers screened, of which 226 bands were polymorphic, and the percentage of polymorphic bands was 36.8%. Observed number of alleles, effective number of alleles, Nei's genetic diversity index and Shannon's information index were 1.992 6, 1.306 5, 0.199 2 and 0.325 1, respectively. Genetic distances of the 21 populations were ranged from 0.039 2 to 0.289 4.</p><p><b>CONCLUSION</b>Species containing extremely aromatic compounds in leaves of Chimonanthus with low genetic diversity play an important role in enhancing the protection of species and germplasm resources. Form the molecular level, the studies demonstrated the correctness of the result by Zhang Ruohui that species containing extremely aromatic compounds in leaves of Chimonanthus were divided into Ch. salicifolius, Ch. Zhejiangensis, Ch. nitens and Ch. grammatus.</p>
Subject(s)
Amplified Fragment Length Polymorphism Analysis , Calycanthaceae , Classification , Genetics , DNA, Plant , Genetics , Genetic Variation , GeneticsABSTRACT
<p><b>OBJECTIVE</b>To reveal the mutative discipline of the contents of the phenols in samples from different provenances.</p><p><b>METHOD</b>The contents of magnolol and honokiol in samples from the filial generation of Guanxi, Hubei and Zhejiang provinces were determined respectively by HPLC.</p><p><b>RESULT</b>There were significant variations between provenances, the contents of honokiol, magnolol and phenols were the highest in samples from Hubei province, those were the lowest in samples from Zhejiang province. There were not only significant differences in polyphenol content between provenances, but also in families in the same provenance and individuals in families.</p><p><b>CONCLUSION</b>Magnolia polyphenol content and appearance traits were significantly related. The type with the small convex tip (original form) were superior to other types of M. officinalis judging from the characteristics of tree classification. The high quality of M. officinalis should actually be attributed to their local varieties and the breeding strategy of M. officinalis should be paid full attention to the good provenances, good families and good individuals.</p>